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type i collagen coated culture dishes  (Thermo Fisher)


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    Structured Review

    Thermo Fisher type i collagen coated culture dishes
    Type I Collagen Coated Culture Dishes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagen+coated+dishes/Collagen/pm41946301-129-3-8
    Average 99 stars, based on 1 article reviews
    type i collagen coated culture dishes - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Gut microbiota‐derived trimethylamine‐N‐oxide inhibits SIRT1 to regulate SM22α‐mediated smooth muscle cell inflammation and promote atherosclerosis progression
    Article Snippet: The supernatant was discarded, and the cells were resuspended in pre‐warmed low‐glucose Dulbecco's Modified Eagle Medium (DMEM) (11965092, Gibco) supplemented with 20% FBS. .. The cells were then seeded onto collagen‐coated dishes and incubated at 37°C with 5% CO 2 in a HeracellTM Vios 160i CO 2 incubator (51033770, Thermo ScientificTM, Germany). ..

    Cell Culture:

    Article Title: Discovery of Thermal Sensitizers That Inhibit Heat-Induced SAFB Granule Formation.
    Article Snippet: HeLa cells (human carcinoma of the cervix) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO) and 1% antibiotic−antimycotic solution (Gibco, Gaithersburg, MD) at 37 °C in an atmosphere of 5% CO2. .. HepG2 cells (human liver cancer) were cultured in Dulbecco’s modified Eagle’s medium (low glucose) (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) in collagen-coated dishes at 37 °C in an atmosphere of 5% CO2. .. H1299 cells (p53-deficient human lung cancer) were cultured in RPMI 1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS and 1% antibiotic−antimycotic solution at 37 °C in an atmosphere of 5% CO2.

    Article Title: Discovery of Thermal Sensitizers That Inhibit Heat-Induced SAFB Granule Formation
    Article Snippet: HeLa cells (human carcinoma of the cervix) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO) and 1% antibiotic–antimycotic solution (Gibco, Gaithersburg, MD) at 37 °C in an atmosphere of 5% CO 2 . .. HepG2 cells (human liver cancer) were cultured in Dulbecco’s modified Eagle’s medium (low glucose) (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) in collagen-coated dishes at 37 °C in an atmosphere of 5% CO 2 . .. H1299 cells (p53-deficient human lung cancer) were cultured in RPMI 1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS and 1% antibiotic–antimycotic solution at 37 °C in an atmosphere of 5% CO 2 .

    Article Title: CircAASS alleviates renal injury and fibrosis by regulating mitochondrial homeostasis in tubular epithelial cells.
    Article Snippet: Renal cortices were dissected into 1-2 mm3 fragments and digested with 0.75 mg/ml collagenase type I (Thermo Fisher Scientific, 17104019) at 37°C for 40 min. .. The suspension was filtered through 100 μm and 40 μm strainers (Fisherbrand, 22-363-549 and 22-363-547) and centrifuged with 31% Percoll (GE Healthcare, 17-0891-01) at 800×g for 20 min. After PBS (Boster, AR0030) washing, cells were cultured in collagen-coated dishes (Thermo Fisher Scientific, A1142801) with DMEM containing 10% FBS, antibiotics, and Insulin-Transferrin-Selenium (Thermo Fisher Scientific, 41400045), maintained at 37°C, 5% CO2. ..

    Article Title: CircAASS alleviates renal injury and fibrosis by regulating mitochondrial homeostasis in tubular epithelial cells
    Article Snippet: Renal cortices were dissected into 1–2 mm 3 fragments and digested with 0.75 mg/ml collagenase type I (Thermo Fisher Scientific, 17104019) at 37°C for 40 min. .. The suspension was filtered through 100 μm and 40 μm strainers (Fisherbrand, 22–363-549 and 22–363-547) and centrifuged with 31% Percoll (GE Healthcare, 17–0891-01) at 800×g for 20 min. After PBS (Boster, AR0030) washing, cells were cultured in collagen-coated dishes (Thermo Fisher Scientific, A1142801) with DMEM containing 10% FBS, antibiotics, and Insulin-Transferrin-Selenium (Thermo Fisher Scientific, 41,400,045), maintained at 37°C, 5% CO2. ..

    Modification:

    Article Title: Discovery of Thermal Sensitizers That Inhibit Heat-Induced SAFB Granule Formation.
    Article Snippet: HeLa cells (human carcinoma of the cervix) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO) and 1% antibiotic−antimycotic solution (Gibco, Gaithersburg, MD) at 37 °C in an atmosphere of 5% CO2. .. HepG2 cells (human liver cancer) were cultured in Dulbecco’s modified Eagle’s medium (low glucose) (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) in collagen-coated dishes at 37 °C in an atmosphere of 5% CO2. .. H1299 cells (p53-deficient human lung cancer) were cultured in RPMI 1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS and 1% antibiotic−antimycotic solution at 37 °C in an atmosphere of 5% CO2.

    Article Title: Discovery of Thermal Sensitizers That Inhibit Heat-Induced SAFB Granule Formation
    Article Snippet: HeLa cells (human carcinoma of the cervix) were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated fetal bovine serum (FBS) (Sigma-Aldrich, St. Louis, MO) and 1% antibiotic–antimycotic solution (Gibco, Gaithersburg, MD) at 37 °C in an atmosphere of 5% CO 2 . .. HepG2 cells (human liver cancer) were cultured in Dulbecco’s modified Eagle’s medium (low glucose) (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) in collagen-coated dishes at 37 °C in an atmosphere of 5% CO 2 . .. H1299 cells (p53-deficient human lung cancer) were cultured in RPMI 1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% heat-inactivated FBS and 1% antibiotic–antimycotic solution at 37 °C in an atmosphere of 5% CO 2 .

    Suspension:

    Article Title: CircAASS alleviates renal injury and fibrosis by regulating mitochondrial homeostasis in tubular epithelial cells.
    Article Snippet: Renal cortices were dissected into 1-2 mm3 fragments and digested with 0.75 mg/ml collagenase type I (Thermo Fisher Scientific, 17104019) at 37°C for 40 min. .. The suspension was filtered through 100 μm and 40 μm strainers (Fisherbrand, 22-363-549 and 22-363-547) and centrifuged with 31% Percoll (GE Healthcare, 17-0891-01) at 800×g for 20 min. After PBS (Boster, AR0030) washing, cells were cultured in collagen-coated dishes (Thermo Fisher Scientific, A1142801) with DMEM containing 10% FBS, antibiotics, and Insulin-Transferrin-Selenium (Thermo Fisher Scientific, 41400045), maintained at 37°C, 5% CO2. ..

    Article Title: CircAASS alleviates renal injury and fibrosis by regulating mitochondrial homeostasis in tubular epithelial cells
    Article Snippet: Renal cortices were dissected into 1–2 mm 3 fragments and digested with 0.75 mg/ml collagenase type I (Thermo Fisher Scientific, 17104019) at 37°C for 40 min. .. The suspension was filtered through 100 μm and 40 μm strainers (Fisherbrand, 22–363-549 and 22–363-547) and centrifuged with 31% Percoll (GE Healthcare, 17–0891-01) at 800×g for 20 min. After PBS (Boster, AR0030) washing, cells were cultured in collagen-coated dishes (Thermo Fisher Scientific, A1142801) with DMEM containing 10% FBS, antibiotics, and Insulin-Transferrin-Selenium (Thermo Fisher Scientific, 41,400,045), maintained at 37°C, 5% CO2. ..

    Isolation:

    Article Title: CES1 Increases Hepatic Triacylglycerol Synthesis Through Activation of PPARγ, LXR and SREBP1c.
    Article Snippet: .. Primary mouse hepatocytes were isolated by collagenase perfusion of livers from chow fed wild-type (C57BL/6J) and Ces1d-/- mice and plated on 60 mm collagen-coated dishes at a confluence of 1.0 × 106 cells/dish, or on #1 coverslips (BD BioCoatTM, Thermo Fisher, Waltham, MA, USA) in six-well plates at 2.0 × 105 cells/well. ..

    Article Title: CES1 Increases Hepatic Triacylglycerol Synthesis Through Activation of PPARγ, LXR and SREBP1c
    Article Snippet: .. Primary mouse hepatocytes were isolated by collagenase perfusion of livers from chow fed wild-type (C57BL/6J) and Ces1d -/- mice and plated on 60 mm collagen-coated dishes at a confluence of 1.0 × 10 6 cells/dish, or on #1 coverslips (BD BioCoat TM , Thermo Fisher, Waltham, MA, USA) in six-well plates at 2.0 × 10 5 cells/well. ..



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